Journal: Renal Failure

Article Title: Baicalin alleviates sepsis-associated acute kidney injury through activation of the PPAR-γ/UCP1 signaling pathway

doi: 10.1080/0886022X.2025.2508908

Figure Lengend Snippet: Baicalin improves LPS-induced kidney damage by reducing inflammation, oxidative stress, and cell apoptosis in renal tissue. (A) Detection of reactive oxygen species levels in renal tissue using DHE staining. (B) Apoptosis was assessed by TUNEL assay. © Observation of macrophage infiltration in renal tissue using F4/80 immunofluorescence staining. (D–F) Quantitative analysis of DHE, TUNEL and F4/80 staining. (G) Western blotting was performed to detect the expression levels of IL-1β, NOX2, Bax and Cleaved-caspase3 proteins. (H–K) Bar graphs showing the expression levels of IL-1β, NOX2, Bax and Cleaved-caspase3 proteins. The data for immunofluorescence are expressed as the means ± SD (n = 6). The data for western blotting are expressed as the means ± SD (n = 4). **p < 0.01 vs. NC group; ##p < 0.01 vs. LPS group

Article Snippet: Primary , cleaved-caspase3 , 9661, CST , 1: 1000.

Techniques: Staining, TUNEL Assay, Immunofluorescence, Western Blot, Expressing

Journal: Renal Failure

Article Title: Baicalin alleviates sepsis-associated acute kidney injury through activation of the PPAR-γ/UCP1 signaling pathway

doi: 10.1080/0886022X.2025.2508908

Figure Lengend Snippet: Baicalin pretreatment prevents LPS-induced inflammatory response and improves mitochondrial function in HK-2 cells. (A–B) Quantitative analysis of DHE and TUNEL staining. (C–E) Representative images showing the oxidative state in HK-2 cells monitored by NAO staining, MitoSOX Red staining and JC-1 staining. Scale bar, 100 μm. (F) Cell viability of LPS-treated HK-2 cells treated with different concentrations of baicalin. (G) LDH of LPS-treated HK-2 cells treated with different concentrations of baicalin. (H–K) Representative images showing the oxidative state in HK-2 cells monitored by DHE staining, TUNEL staining, NAO staining and MitoSOX Red staining. Scale bar, 100 μm. (L–O) Bar graphs showing the expression levels of IL-1β, NOX2, BAX and Cleaved-caspase3 proteins. (P) Western blotting was performed to detect the expression levels of IL-1β, NOX2, BAX and Cleaved-caspase3 proteins. The data for immunofluorescence are expressed as the means ± SD (n = 6). The data for western blotting are expressed as the means ± SD (n = 4). **p < 0.01 vs. NC group; ##p < 0.01 vs. LPS group.

Article Snippet: Primary , cleaved-caspase3 , 9661, CST , 1: 1000.

Techniques: TUNEL Assay, Staining, Expressing, Western Blot, Immunofluorescence

Journal: Renal Failure

Article Title: Baicalin alleviates sepsis-associated acute kidney injury through activation of the PPAR-γ/UCP1 signaling pathway

doi: 10.1080/0886022X.2025.2508908

Figure Lengend Snippet: After knocking down PPAR-γ/UCP1, baicalin lost its inhibitory effect on IL-1β, NOX2, BAX, and cleaved-caspase3 in the LPS-induced HK2 cell injury model. (A–B) Expressions of IL-1β, NOX2, BAX and Cleaved-caspase3 through western blotting. (C–R) Bar graphs showing the expression levels of IL-1β, NOX2, BAX and Cleaved-caspase3. The data are expressed as the means ± SD (n = 4). **p < 0.01 vs. Con group; ##p < 0.01 vs. LPS group;NS: No statistical significance.

Article Snippet: Primary , cleaved-caspase3 , 9661, CST , 1: 1000.

Techniques: Western Blot, Expressing